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consort e835 manual

consort e835 manual

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consort e835 manual

CONSORT will repair all defective equipment returned to it during the warranty period without charge, provided the equipment has been used under normal laboratory conditions and in accordance with the operating limitations and maintenance procedures in this instruction manual and when not having been subject to accident, alteration, misuse or abuse. A return authorisation must be obtained from CONSORT before returning any product for warranty repair on a freight prepaid basis. CONSORT certifies that this instrument was thoroughly inspect-ed and tested at the factory prior to shipment and found to meet all requirements defined by contract under which it is furnished. However, dimensions and other physical characteristics may differ.The normal operating temperature should be between 4 and 40C. Never store the instrument in a room with high humidity or at very low temperatures (con-densation water!).Connect the instrument only to an earthed power line. The required power source is indicated on the label at the back of the instrument. Do not cut and splice the power cord. When removing the power cord from the wall outlet, be sure to unplug by holding the plug attachment and not by pulling the cord. Do not hold the plug by wet hand. A return authorisation must be obtained from CONSORT before returning any product for warranty repair on a freight prepaid basis!CONSORT is not liable for consequential damages arising out of the use or handling of its products. ServicingIn the event of this instrument being returned for servicing, the owner is request-ed to remove the power supply lead and NOT to send the following items unless they are suspect:ManualCablesAccessoriesIf serious malfunctioning occurs, stop using the unit immediately and consult your local CONSORT dealer. Connected tank has a too low resist- ance (check thoroughly the complete assembly). This instrument is equipped with safety outputs on which the high voltage cannot suddenly appear.
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The built in microcomputer will always smoothly increase the voltage till one of the pre-set limits is reached. The unit is fully protected against any overload condition by a special safety system which automatically disconnects the AC line from the high voltage transformer. A flashing error code in the display and an acoustic alarm is activated whenever a dangerous condition is present. As it is capable of giving dangerous voltage levels by which high power is involved, we suggest that you take a few moments to read this manual thoroughly. Although this instrument is equipped with all necessary safety features against abuse and other accidental failures, caution should be exercised when working with high voltage equipment. Therefore, avoid to touch the outlets with any conducting object and make sure there is a second person present for your safety in case of any severe electric shock. Never touch any part of the assem-bly (power supply, leads or tank) before having switched off. Never manipulate with wet hands. Do not ground any of the outputs or the buffer in the tank. Connect the outlets only to an insulated electrophoresis tank with safety cover. Never make any other connections, such as e.g. putting several power supplies in series or in parallel. In order to prevent electric shock, never open the back plate nor remove the cover. Do not expose the unit to rain or any other liquid. This range of high voltage power supplies from Consort meets all modern In Manual mode you set the voltage, current and time, and you can change these When supplied 220vac this unit power is on. Unit is Used but in good physical condition.Horizontal Buffer Saver This Consort Mini power supply covers the ideal voltage range generally. Looks OK physically. ” This includes items that are defective in ways that render them difficult to use, items that require service or repair, or items missing essential components. See the seller's listing for full details. Looks OK physically.
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” Please include, as a note, your preferred shipping method. This should be added to Mail or PAYPAL payments. If you are an international bidder please ASK for exact actual shipping cost. Please refer to the US Postal Service as the Post Office, I have had confusions with the terms USPS and UPS. If no note is included I will ship cheapest, usually slowest way. The item may have some signs of cosmetic wear, but is fully operational and functions as intended. This item may be a floor model or store return that has been used. See the seller’s listing for full details and description of any imperfections. Please include, as a note, your preferred shipping method. Consort Electrophoresis Power Supply EV2310 EV2000 series is a high-end mid-power range suitable for most applications such as larger tanks or multiple smaller tanks. A robust 150W power supply in a small housing designed to be easy to use. EV2310 (300V, 1000mA): an excellent choice for blotting, multiple horizontal and vertical gels. The front panel and graphical display are designed for ease of use. The display provides all useful information during runs and will show an on screen help to guide the user in setting up the power supply. In Simple Mode you just have to set your power supply tothe desired parameters and press run. EV2000 series has a firmware upgrade capability so future improvements and features will always be available. Moreover EV2000 series has continuous logging combined with a real time clock so it’s possible to get an overview of previous runs, including possible down-times in case of mains power failures. The complete EV series can keep it’s voltage constant at low currents without problem and will keep on functioning at low and high temperatures. Super high amount of views. 0 sold, 1 available. More Super high amount of views. 0 sold, 1 available. You are the light of the world.

Array Comparative Genomic Hybridization: Protocols and Applications explores the scope of what is now possible as far as documenting abnormalities associated with several human cancers. While the technology for interrogating the human genome continues to evolve, refinement of comparative genomic hybridization (CGH) using array CGH and related technologies have provided enormous insight into human cancers at an affordable scale. Authoritative and easily accessible, Array Comparative Genomic Hybridization: Protocols and Applications provides researchers with well-honed methodologies to learn these techniques for their own use in research or clinical diagnostic laboratories. Connectez-vous pour afficher vos informations Mon eBay Developper Mon eBay Recapitulatif Recemment consultes Encheres en cours Affaires a suivre Objets achetes Mes ventes Recherches favorites Vendeurs favoris Messages Notification Developper le panier Chargement en cours. Un probleme est survenu. Pour en savoir plus, affichez le panier. Looks OK physically. ” En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet Ce montant pourra etre modifie jusqu'a ce que vous ayez effectue le paiement. Pour en savoir plus, consultez les Conditions d'utilisation du Service de livraison internationale - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet Ce montant pourra etre modifie jusqu'a ce que vous ayez effectue le paiement. Si vous residez dans un pays membre de l'Union europeenne autre que le Royaume-Uni, vous ne pourrez pas recuperer la TVA a l'importation pour cet achat.
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Pour en savoir plus, consultez les Conditions d'utilisation du Service de livraison internationale - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet Les delais de livraison peuvent varier, notamment pendant les periodes de pointe, et dependent du lieu ou se trouve l'acheteur En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet Cette categorie inclut les objets defectueux et donc difficiles a utiliser, les objets necessitant un entretien ou une reparation ou les objets dont les composants principaux sont manquants. Consulter l'annonce du vendeur pour avoir plus de details. Looks OK physically. ” Contactez le vendeur - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet pour connaitre les modes de livraison disponibles vers votre destination. Saisissez un code postal valide. Saisissez un chiffre inferieur ou egal a 1. Les delais de livraison peuvent varier, notamment pendant les periodes de pointe. Cependant, il n'existe aucune garantie legale de conformite des objets. Les achats aupres de vendeurs non professionnels ne beneficient pas du droit de retractation. Si le vendeur accepte les retractations, cette information sera indiquee dans l'annonce. En savoir plus sur les garanties legales sur eBay Pour les transactions repondant aux conditions requises, vous etes couvert par la Garantie client eBay si l'objet que vous avez recu ne correspond pas a la description fournie dans l'annonce. Please include, as a note, your preferred shipping method. If no note is included I will ship cheapest, usually slowest way. Certains achats specifiques ne sont pas couverts par cette garantie. En savoir plus.

Tous droits reserves. Conditions d'utilisation, Donnees personnelles, cookies et AdChoice Norton Secured - developpe par Verisign. Pour en savoir plus, affichez le panier. En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet Ce montant pourra etre modifie jusqu'a ce que vous ayez effectue le paiement. Pour en savoir plus, consultez les Conditions d'utilisation du Service de livraison internationale - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet Les delais de livraison peuvent varier, notamment pendant les periodes de pointe, et dependent du lieu ou se trouve l'acheteur En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet En savoir plus - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet Objet presentant quelques marques d'usure superficielle, entierement operationnel et fonctionnant correctement. Il peut s'agir d'un modele de demonstration ou d'un objet retourne en magasin apres une courte periode d'utilisation. Consulter la description du vendeur pour avoir plus de details sur les eventuelles imperfections. Contactez le vendeur - la page s'ouvre dans une nouvelle fenetre ou un nouvel onglet pour connaitre les modes de livraison disponibles vers votre destination. Saisissez un code postal valide. Saisissez un chiffre inferieur ou egal a 1. Les delais de livraison peuvent varier, notamment pendant les periodes de pointe. Cependant, il n'existe aucune garantie legale de conformite des objets. The current paper investigates whether communicating evidence of a policy's effectiveness at tackling the focal problem could increase support.

Across three studies we develop an infographic for communicating quantitative evidence of the effectiveness of a hypothetical tax to tackle childhood obesity. In Study 1, communicating evidence did not change support for the policy. Variations of the infographic were developed in Study 2 with one emerging as clearer and easier to comprehend. This infographic was therefore used in Study 3 in which it increased support for the tax from 45 to 49, an effect that was mediated by perceived effectiveness. The effect sizes were small but probably meaningful at a population level. The results of these three studies suggest the potential for presenting quantitative evidence of intervention effectiveness to increase public support. Much uncertainty remains about the most effective ways of presenting this evidence, whether similar effects are achieved by presenting unquantified evidence and whether larger effects might be achieved by presenting information other than effectiveness. Previous article in issue Next article in issue Keywords Public health Policy Communication Updating beliefs Belief change Attitudes Recommended articles Citing articles (0). The authors are grateful to useful advice and critical comments from Kaidy Stautz, Robbie Duschinsky, Mike Kelly, Katie Saunders, Mike Pearson, David Spiegelhalter, Alex Freeman, Sander van der Linden, Cameron Brick, and Eleni Mantzari. Published by Elsevier Ltd. Recommended articles No articles found. Citing articles Article Metrics View article metrics About ScienceDirect Remote access Shopping cart Advertise Contact and support Terms and conditions Privacy policy We use cookies to help provide and enhance our service and tailor content and ads. By continuing you agree to the use of cookies. Advanced G'day ( Sign in to bid or buy) eBay Deals Coles on eBay Help Sell Watch List Expand Watch list Loading. Something went wrong.User Agreement, Privacy, Cookies and AdChoice Norton Secured - powered by Verisign.

Popis pristroje Pohled zepredu a z boku Seznam soucasti A. Tryska B. Kryt vodni nadrzky C. Nahradni Ma jak vysokou stabilitu analogoveho Prosim, dukladne si prectete manual a dbejte na nasledujici pokyny, aby se zabranilo skodam na zarizeni. Za skody Pred pouzitim pristroje si prosim peclive prectete tento Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod Prectete si pozorne prirucku a dodrzujte nasledujici instrukce, abyste predesli poskozeni. Jakekoliv poruchy zpusobene ignorovanim instrukci Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod Pred pouzitim produktu si dukladne prectete tuto prirucku, abyste mohli plne Dovozce do CR SATURN HOME APPLIANCES s.r.o. Web: Gratulujeme Vam ke koupi vyrobku od znacky Saturn. Prosim, dukladne si pozorne manual a dbejte na nasledujici pokyny, aby se zabranilo skodam na zarizeni.Pristroj nesmi byt vystaven kapajici nebo strikajici kapaline Gratulujeme Vam ke koupi vyrobku od znacky Saturn. Jsme si jisti, ze Prosim, dukladne si prectete manual a dbejte na nasledujici pokyny k montazi, aby se zabranilo skodam na zarizeni. Obsahuje dulezite pokyny k uvedeni pristroje do provozu a k jeho obsluze. Jestlize vyrobek Prosim, dukladne si pozorne manual a dbejte na nasledujici pokyny, aby se zabranilo skodam na zarizeni. Za skody zpusobene Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod uschovejte Dovozce do CR SATURN HOME APPLIANCES s.r.o. Web: Gratulujeme Vam ke koupi vyrobku od znacky Saturn. Jsme si jisti, Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod uschovejte pro pripad Navod k pouziti Dovozce do CR SATURN HOME APPLIANCES s.r.o. Web: Gratulujeme Vam ke koupi vyrobku od znacky Saturn. Jsme si jisti, Aby se zabranilo moznemu technickemu poskozeni, prosime Vas, Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti.

Navod uschovejte Nepokladejte predmety naplnene tekutinou, napriklad Obsahuje dulezite pokyny pro bezpecnou instalaci, obsluhu a udrzbu. Uschovejte tyto pokyny pro pripad pristiho Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod uschovejte Pristroj nesmi byt vystaven kapajici nebo strikajici kapaline Dohlednete na to, aby byl privodni kabel polozen mimo horke casti pristroje a aby pristroj nebyl nijak Pred pouzitim si pozorne prectete tento navod. V opacnem pripade riskujete ohrozeni sveho zdravi a poskozeni pristroje. Dovozce do CR SATURN HOME APPLIANCES s.r.o. Web: Gratulujeme Vam ke koupi vyrobku od znacky Saturn. Jsme si jisti, ze Aby se zabranilo moznemu technickemu poskozeni, prosime Vas, abyste si pozorne precetli tento Gratulujeme Vam ke koupi vyrobku od znacky Saturn. Jsme si jisti, Prosim, dukladne si prectete manual a dbejte na nasledujici pokyny, aby se zabranilo skodam na zarizeni. Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod uschovejte Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod uschovejte pro Prosim, prectete si tuto prirucku, abyste se ujistili, Uzivatelsky manual Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod uschovejte Pred pripojenim zarizeni k napajecimu zdroji Zajisteni odolnosti vuci vode a prachu. Ikony v pokynech Obrazky se mohou od vzhledu konkretniho produktu lisit. Obsah Navod k obsluze Navod k obsluze Obsahuje dulezite pokyny pro bezpecnou instalaci, obsluhu a udrzbu. Navod uschovejte Prirucka uzivatele Tato prirucka obsahuje dulezite bezpecnostni a provozni Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti.

Navod uschovejte Aby se zabranilo moznemu technickemu poskozeni, prosime Vas, abyste si pozorne precetli tento Pri nedodrzeni pokynu muze dojit ke zranenim Aby vas spotrebic slouzil co nejlepe, prectete si vsechny pokyny v tomto navodu. OBSAH Varic ryze model c.g10g01 Zhruba dodrzte pomer: AX-3005DBL-jednokanalovy AX-3005DBL-3-trikanalovy. Navod k obsluze Prosim, dukladne si prectete manual a dbejte na nasledujici pokyny, aby se zabranilo skodam na zarizeni. Spotrebic pouzivejte pouze tak, jak je popsano v tomto navodu k pouziti. Navod uschovejte Prosim, dukladne si prectete manual a dbejte na nasledujici pokyny, aby se zabranilo skodam na zarizeni. Za Pri manipulaci dbejte bezpecnostnich zasad pouziti elektronickych spotrebicu. Prectete si, prosim, pozorne Gratulujeme Vam ke koupi vyrobku od znacky Saturn. Jsme si jisti, ze nas vyrobek se Dovozce do CR SATURN HOME APPLIANCES s.r.o. Web: Gratulujeme Vam ke koupi vyrobku od znacky Saturn. Jsme si jisti, Prosim, dukladne si prectete manual a dbejte na nasledujici pokyny, aby se zabranilo skodam na zarizeni. Nez jej zacnete pouzivat, prectete si prosim navod k obsluze. Nez jej zacnete pouzivat, prectete si prosim navod k obsluze. 2. Bezpecnost Navod k obsluze obsahuje dulezite V dusledku toho mohou byt jeji vykonove vystupy pod napetim, i kdyz je Dovozce do CR SATURN HOME APPLIANCES s.r.o. Web: Gratulujeme Vam ke koupi vyrobku od znacky Saturn. Jsme To use this website, you must agree to our Privacy Policy, including cookie policy. Search for more papers by this author Published Online: 01 May 2013 This is the final version - click for previous version More Sections PDF (160 KB) Download PDF Our aim was to investigate whether acupuncture affects ovulation frequency and to understand the underlying mechanisms of any such effect by analyzing LH and sex steroid secretion in women with PCOS. This prospective, randomized, controlled clinical trial was conducted between June 2009 and September 2010.

Secondary outcomes were changes in the secretion of sex steroids, anti-Mullerian hormone, inhibin B, and serum cortisol. Ovulation frequency during treatment was higher in the acupuncture group than in the control group. Ovarian and adrenal sex steroid serum levels were reduced with no effect on LH secretion. PCOS is related to hyperinsulinemia and insulin resistance and is exacerbated by obesity ( 7 ). Numerous studies have reported hypersecretion of luteinizing hormone (LH) in women with PCOS ( 3 ). Together with an exaggerated ovarian response, hypersecretion of LH drives excessive ovarian androgen production and causes anovulation ( 13 ). In PCOS, altered sex steroid production, metabolic dysfunction, and obesity all contribute to changes in LH secretion patterns and to anovulation ( 6, 12, 29, 30 ). Clomiphene citrate, exogenous gonadotropin therapy, and laparoscopic ovarian drilling are commonly used to induce ovulation in women with PCOS ( 1 ). These treatments often have negative side effects, thus indicating the importance of evaluating alternative treatments such as acupuncture. Acupuncture is used worldwide to achieve fertility, but its efficacy is supported by only limited scientific evidence. In a randomized controlled trial (RCT), we previously demonstrated that acupuncture with manual and low-frequency electrical needle stimulation was superior to both exercise and no treatment for improving menstrual frequency and total testosterone levels ( 15 ). The effect of acupuncture was, at least in part, mediated by reduction in sympathetic nerve activity ( 39 ). The main limitations of that study were the inability to confirm ovulation and to control for the increased attention associated with the therapeutic meeting and needle insertion. In another RCT, true acupuncture was compared with sham acupuncture using “placebo needles” ( 31 ).

The ovulation frequency did not differ between the groups, and both groups showed improved LH-to-follicle-stimulating hormone (FSH) ratios. The lack of a difference between true and sham acupuncture in that study is in line with previous studies on different pain conditions and nausea caused by chemotherapy that demonstrated that true acupuncture is not more effective than sham acupuncture. However, all of these trials found a significant effect when groups were compared with a nonintervention group ( 8, 22, 28 ). These results indicate that sham acupuncture is not an inert method and highlight the methodological difficulties in the design of acupuncture trials. To compensate for the attention that women treated by acupuncture would receive, a control group was randomized to meet with a therapist, because sham acupuncture has been shown not to be an inert control. The primary outcome measures were changes in LH pulsatility from baseline to the end of treatment and differences in ovulation frequency during the study. MATERIALS AND METHODS Study Population PCOS patients were recruited by advertisements in local newspapers between June 2009 and September 2010. A self-reported Ferriman-Gallwey (FG) score of ?8 defined the presence of hirsutism, and the presence of acne was defined by an affirmative answer to being asked if they had excessive acne. The study was registered at ClinicalTrials.gov (NTC00921492), and is reported according to the CONSORT and STRICTA guidelines ( 24, 35 ). Participants recorded menstrual bleeding patterns, and ovulation in all women was confirmed by weekly progesterone measurements throughout the study. Participants were randomized before baseline assessments to either receiving acupuncture or to receiving similar attention by meeting with a therapist. Randomization was computer generated ( ), and investigators were blinded until statistical analyses.

After randomization, each participant underwent a 10- to 13-wk intervention period after which the baseline measurements were repeated. Baseline assessments were made at the Endocrine and Metabolic Research Centre at Sahlgrenska during an overnight stay. Power Calculation and Sample Size Power calculation and sample size were determined on the basis of changes in the 12-h LH pulse frequency in women with PCOS from baseline to end of treatment. We expected a 10 change in the number of pulses with a mean change (?) of ?0.8 and standard deviation (SD) of 1.81 within the groups ( 32 ). A total of 14 patients per group was required for a statistical power of 80 at a 5 significance level. We enrolled 16 subjects per group to account for dropouts. Interventions Acupuncture. Subjects rested and listened to relaxing music during treatment. The acupuncture protocol was based on a previous study of acupuncture for ovulation induction in PCOS ( 15 ), an experimental study ( 37 ), and clinical experience. Two sets of 11 and 13 acupuncture points were alternated every other treatment due to the intensity of the treatment ( Table 1 ). All needles were rotated manually to evoke needle sensation (de qi) when inserted. Needles in the leg and abdominal muscles were then connected to an electrical stimulator (CEFAR ACUS 4; Cefar-Compex Scandinavia, Landsbro, Sweden) and stimulated with low-frequency (2 Hz) bursts. The intensity was adjusted to produce local muscle contractions without pain or discomfort.Attention control. Women in the control group visited the same physical therapists. Time was spent resting and listening to relaxing music, with the therapist entering the room every 10 min ensuring comfort and well-being in a manner similar to the acupuncture group. Outcome Measurements All measurements were taken at baseline and were repeated within 1 wk after the last treatment (referred to as end of treatment in results ).

If no ovulation or bleeding had occurred during the period between screening for the study and the taking of the baseline measurements, the measurements were taken on an arbitrary day of the cycle. Outcome measurements. A foot-to-foot bioelectrical impedance system (Tanita, Middlesex, UK) was used to measure the percent body fat. Hirsutism was assessed by the FG score. Ovarian volume, endometrial thickness, and antral follicle ( Immunoassay LH, FSH, AMH, inhibin B, glucose, cholesterol (total, HDL, and LDL), insulin, progesterone, and SHBG were analyzed at an accredited laboratory at the Department of Clinical Chemistry, Sahlgrenska University Hospital. All of these were measured with kits from Roche Diagnostics (Mannheim, Germany) on a Cobas instrument. Mass Spectrometry Circulating concentrations of sex steroids, androgen precursors, and glucuronidated androgen metabolites were analyzed by mass spectrometry. Analyses were performed by the Endoceutics Bioanalytical Laboratory (Quebec, Canada) essentially as described ( 18, 19 ). Free testosterone (free T) was calculated with the spreadsheet developed by Mazer et al. ( 27 ) where cortisol and cortisol-binding globulin were set to zero. The area under the curve (AUC) for all sex steroid concentrations, measured every 4th hour from 0 to 12 h, was calculated using the linear trapezoidal method. Gas Chromatography with Detection by Tandem Mass Spectrometry Total deuterated estradiol (E 2 ), total testosterone (T), androstenedione (4-DIONE), dehydroepiandrosterone (DHEA), total estrone (E 1 ), and total dihydrotestosterone (DHT) were added as internal standards (CDN Isotopes, Essex, UK) to 0.5-ml serum samples to enable quantification of their respective free steroids. Ultraperformance Liquid Chromatography Tandem Mass Spectrometry Deuterated androstane-3?,17?-diol-3-glucuronide (AD3G), androstane-3?,17?-diol-17-glucuronide (AD17G), and androsterone glucuronide (ADTG) were added as internal standards to 400-?

l serum samples to enable quantification of their respective glucuronidated steroids. Deuterated DHEA sulfate (DHEA-S) and E 1 sulfate (E 1 -S) were added as internal standards to 75-?l serum samples to enable quantification of their respective sulfated steroids and were extracted as above. Data Analysis Hormone pulsatility. The concentrations of LH and cortisol in blood samples taken every 10 min for 12 h were analyzed with the Autodecon pulse detection algorithm ( 17 ) in the Pulse XP software package to detect and quantify secretory events. Three truncated LH data series, due to missing blood samples, were recalculated to 12 h for the time-dependent variables before statistical analysis. This recalculation was applied to one baseline series in the acupuncture group and to one end-of-treatment series in both the acupuncture and control groups. Two truncated cortisol data series in the acupuncture group, one baseline, and one end of treatment, were recalculated to 12 h for the time-dependent variables. Quantification of irregularity. To quantify the regularity or randomness of the LH data series, we analyzed the time courses with the Approximate Entropy (ApEn) application in the Pulse XP software package. ApEn is a statistical method that, in contrast to conventional pulse-detection procedures, evaluates both dominant and subdominant patterns in time series that are not reflected in peak occurrences or amplitudes. Larger values represent greater randomness or irregularity, and smaller numbers represent more recognizable patterns ( 44 ). One thousand Monte Carlo simulations were used to calculate the standard deviation of approximate entropy in each series. Statistical Analyses Between-group differences for baseline values and the change between baseline and end of treatment (.The ? 2 test was used to assess differences in acne and menstrual cycle pattern (categorical variables) between groups at baseline.